136632-95-6 , 4-Nitrophenyl a-nigeroside; 4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside

136632-95-6 , 4-Nitrophenyl a-nigeroside;
4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside,
Cas:136632-95-6
C18H25NO13 / 463.392
MFCD04039649

4-Nitrophenyl a-nigeroside

4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside

4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside (pNP-Glc-Glc) is a highly sensitive and potent chromogenic substrate for the enzymatic detection and measurement of alpha-glucosidase activity. The rapid color change triggered by the hydrolysis of pNP-Glc-Glc provides a clear and efficient method for monitoring enzyme kinetics, screening inhibitors, and functional characterization of Î±-glucosidases. This substrate is soluble in aqueous and polar organic solvents and yields a detectable 4-nitrophenol product with a distinct yellow color under both visible and ultraviolet light, enabling quantitative spectrophotometric measurements. With a superior signal-to-noise ratio and maximal sensitivity, pNP-Glc-Glc is a reliable and robust option for biochemical and biomedical research involving glucosidase enzymes.

CAS Number	136632-95-6
Product Name	4-Nitrophenyl 3-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside
IUPAC Name	(2R,3R,4S,5S,6R)-2-[(2R,3R,4S,5R,6R)-3,5-dihydroxy-2-(hydroxymethyl)-6-(4-nitrophenoxy)oxan-4-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol
Molecular Formula	C18H25NO13
Molecular Weight	463.392
InChI	InChI=1S/C18H25NO13/c20-5-9-11(22)13(24)14(25)17(30-9)32-16-12(23)10(6-21)31-18(15(16)26)29-8-3-1-7(2-4-8)19(27)28/h1-4,9-18,20-26H,5-6H2/t9-,10-,11-,12-,13+,14-,15-,16+,17-,18+/m1/s1
InChI Key	LBTDRWMZFQVCAR-SJBMRWFPSA-N
SMILES	C1=CC(=CC=C1[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)OC3C(C(C(C(O3)CO)O)O)O)O
Synonyms	4-Nitrophenyl α-Nigeroside; 4-Nitrophenyl 3-O-α-D-Glucopyranosyl-α-D-glucopyranoside;