157843-41-9, 2-Chloro-4-nitrophenyl α-L-fucopyranoside, CAS:157843-41-9

157843-41-9, 2-Chloro-4-nitrophenyl α-L-fucopyranoside,
CAS:157843-41-9
C12H14ClNO7 / 319.69
MFCD04972056

2-Chloro-4-nitrophenyl a-L-fucopyranoside

Chromogenic substrate for alpha-L-Fucosidase. The AFU assay kit is based on the enzymatic cleavage of the synthetic substrate CNPF to α-L-fucose and 2-chloro-4-nitrophenol, which is quantified by measuring the absorbances at 405 nm in a kinetic fashion. It is a one step assay with a single assay reagent. One unit of AFU is defined as the amount of AFU that cleaves one μmole of 2-chloro-4-nitrophenyl-α-L-fucoside per min at 37 °C.

CAS Number	157843-41-9
Product Name	2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside
IUPAC Name	(2S,3S,4R,5S,6S)-2-(2-chloro-4-nitrophenoxy)-6-methyloxane-3,4,5-triol
Molecular Formula	C₁₂H₁₄ClNO₇
Molecular Weight	319.69 g/mol
InChI	InChI=1S/C12H14ClNO7/c1-5-9(15)10(16)11(17)12(20-5)21-8-3-2-6(14(18)19)4-7(8)13/h2-5,9-12,15-17H,1H3/t5-,9+,10+,11-,12-/m0/s1
InChI Key	QURSGHQPKUXLAD-MOBXTKCLSA-N
SMILES	CC1C(C(C(C(O1)OC2=C(C=C(C=C2)[N+](=O)[O-])Cl)O)O)O
Synonyms	2-chloro-4-nitrophenyl alpha-L-fucopyranoside, 2-Cl-4-NP-F
Canonical SMILES	CC1C(C(C(C(O1)OC2=C(C=C(C=C2)[N+](=O)[O-])Cl)O)O)O
Isomeric SMILES	C[C@H]1[C@H]([C@H]([C@@H]([C@@H](O1)OC2=C(C=C(C=C2)[N+](=O)[O-])Cl)O)O)O

CAS No: 157843-41-9 MDL No: MFCD04972056 Chemical Formula: C12H14ClNO7 Molecular Weight: 319.69

COA:

Name: 2-Chloro-4-nitrophenyl-alpha-L-fucopyranoside; CNP-AFU; CNPF

CAS:157843-41-9 M.F.: C₁₂H₁₄ClNO₇M.W._:319.69

*Items*	*Standards*	*Results*
Appearance	White crystal powder	Complies
Solubility	Soluble in DMF, insoluble in petroleum ether	Complies
Identification	IR and HPLC	Complies
MS and NMR	Should comply	Complies
Melting point	150℃ ~ 160 °C	155℃ ~158 ℃
Specific rotation ( [α]^20/D)	-163^o ~ -173^o	-172.5^o
Any other impurity	Max. 0.5%	Complies
Heavy metal	Max. 20ppm	Complies
Residue on ignition	Max. 0.5%	0.1%
TLC	Should be one spot	One spot
Assay by HPLC	Min. 98%	99.2%