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  • 1041195-59-8 , 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside
1041195-59-8 , 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside

1041195-59-8 , 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside

1041195-59-8 , 4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside
Cas:1041195-59-8
C32H41NO17 / 711.66
MFCD04039650

4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside

4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-4,6-O-cyclohexylidene-b-D-mannopyranoside is a chromogenic pNP enzyme substrate, which features a cyclohexylidene-protected mannopyranosyl core. Upon enzymatic hydrolysis by glycosidases or other related enzymes, it releases a yellow-colored product, 4-nitrophenol, that can be easily detected spectrophotometrically. This substrate is useful for studying enzyme specificity, kinetics, and inhibition, as well as for the screening of enzyme inhibitors, the identification of glycosidase-producing organisms, and the development of new analytical methods.

CAS Number1041195-59-8
Product Name4-Nitrophenyl 3-O-(2,3,4,6-tetra-O-acetyl-alpha-D-mannopyranosyl)-4,6-O-cyclohexylidene-beta-D-mannopyranoside
IUPAC Name[(2R,3R,4S,5S,6R)-6-[(4aR,6S,7S,8R,8aR)-7-hydroxy-6-(4-nitrophenoxy)spiro[4,4a,6,7,8,8a-hexahydropyrano[3,2-d][1,3]dioxine-2,1'-cyclohexane]-8-yl]oxy-3,4,5-triacetyloxyoxan-2-yl]methyl acetate
Molecular FormulaC32H41NO17
Molecular Weight711.66 g/mol
InChIInChI=1S/C32H41NO17/c1-16(34)41-14-22-25(43-17(2)35)28(44-18(3)36)29(45-19(4)37)31(48-22)49-27-24(38)30(46-21-10-8-20(9-11-21)33(39)40)47-23-15-42-32(50-26(23)27)12-6-5-7-13-32/h8-11,22-31,38H,5-7,12-15H2,1-4H3/t22-,23-,24+,25-,26-,27-,28+,29+,30-,31-/m1/s1
InChI KeyPFVIMOHQGFNKCN-JXKBUMJTSA-N
SMILESCC(=O)OCC1C(C(C(C(O1)OC2C(C(OC3C2OC4(CCCCC4)OC3)OC5=CC=C(C=C5)[N+](=O)[O-])O)OC(=O)C)OC(=O)C)OC(=O)C
Canonical SMILESCC(=O)OCC1C(C(C(C(O1)OC2C(C(OC3C2OC4(CCCCC4)OC3)OC5=CC=C(C=C5)[N+](=O)[O-])O)OC(=O)C)OC(=O)C)OC(=O)C
Isomeric SMILESCC(=O)OC[C@@H]1[C@H]([C@@H]([C@@H]([C@H](O1)O[C@@H]2[C@@H]([C@@H](O[C@H]3[C@H]2OC4(CCCCC4)OC3)OC5=CC=C(C=C5)[N+](=O)[O-])O)OC(=O)C)OC(=O)C)OC(=O)C


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