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  • 1956-11-2 ,4-硝基苯基月桂酸酯,4-Nitrophenyl laurate;4-Nitrophenyl dodecanoate
1956-11-2 ,4-硝基苯基月桂酸酯,4-Nitrophenyl laurate;4-Nitrophenyl dodecanoate

1956-11-2 ,4-硝基苯基月桂酸酯,4-Nitrophenyl laurate;4-Nitrophenyl dodecanoate

1956-11-2 , 4-硝基苯基月桂酸酯,
4-Nitrophenyl laurate;4-Nitrophenyl dodecanoate,
Cas:1956-11-2
C18H27NO4 / 321.41
MFCD00047730

4-Nitrophenyl laurate;4-Nitrophenyl dodecanoate

4-硝基苯基月桂酸酯

4-Nitrophenyl laurate is a chemical compound that has been shown to inhibit the activity of enzymes. It binds to the active site of an enzyme, blocking its access to the substrate and preventing catalysis. This effect may be due to the hydrophobic nature of 4-nitrophenyl laurate, which creates a hydrophobic effect that prevents other molecules from binding. 4-Nitrophenyl laurate has been shown to inhibit some enzymes in vitro, but it is not yet known how it can affect biological properties or if it inhibits transcriptional regulation.

4-nitrophenyl laurate (4-NPL) is an organic compound with the molecular formula C13H17NO4. This compound is widely used in scientific research as a substrate for lipase and esterase activity assays. In this paper, we will explore the various aspects of 4-NPL, including its definition and background, physical and chemical properties, synthesis and characterization, analytical methods, biological properties, toxicity and safety in scientific experiments, applications in scientific experiments, current state of research, potential implications in various fields of research and industry, limitations, and future directions.

Definition and Background

4-nitrophenyl laurate (4-NPL) is a member of the 4-nitrophenyl ester family of compounds, which are widely used as substrates for lipase and esterase assays. This compound consists of a 4-nitrophenyl group attached to a laurate moiety. The laurate moiety is a long-chain fatty acid with 12 carbon atoms, and the 4-nitrophenyl group is a chromophore that absorbs light at around 400 nm.

Physical and Chemical Properties

4-NPL is a pale yellow crystalline solid that is sparingly soluble in water and soluble in organic solvents like ethanol, methanol, and acetonitrile. The compound has a melting point of 78-81°C and a molecular weight of 247.29 g/mol. The chemical structure of 4-NPL is shown below.

Synthesis and Characterization

4-NPL can be synthesized by reacting 4-nitrophenol with lauric acid and a dehydration agent like thionyl chloride or phosphorus pentoxide. The reaction results in the formation of 4-nitrophenyl laurate and a byproduct like hydrochloric acid or phosphoric acid.

The synthesized 4-NPL can be characterized by several techniques like NMR spectroscopy, mass spectrometry, and UV-vis spectroscopy. NMR spectroscopy can be used to identify the chemical shifts of the protons in the compound, while mass spectrometry can be used to determine the molecular weight and fragmentation pattern of the compound. UV-vis spectroscopy can be used to measure the absorbance of the compound at different wavelengths and to determine the molar extinction coefficient.

Analytical Methods

4-NPL is widely used as a substrate for lipase and esterase activity assays. The hydrolysis of 4-NPL by lipases and esterases results in the release of 4-nitrophenol, which can be easily quantified by spectrophotometric methods. The absorbance of 4-nitrophenol at 405 nm can be used to determine the rate of hydrolysis and the specific activity of the enzyme.

Biological Properties

4-NPL is not known to have any biological activity itself, but it is widely used in biochemistry and molecular biology to measure the activity of lipases and esterases. These enzymes are involved in the metabolism of lipids and play important roles in various physiological processes like digestion, energy homeostasis, and signal transduction.

Toxicity and Safety in Scientific Experiments

4-NPL is not known to be toxic to humans or animals at the concentrations used in scientific experiments. However, like all chemicals, it should be handled with care and proper safety precautions should be taken to avoid exposure to the eyes, skin, or mucous membranes.

Applications in Scientific Experiments

4-NPL is widely used as a substrate for lipase and esterase activity assays in biochemistry and molecular biology. The assay can be used to determine the activity of lipases and esterases in different biological samples like plasma, serum, tissue homogenates, and cell lysates. The assay can also be used to screen for potential inhibitors or activators of these enzymes.

Current State of Research

There is a significant amount of research focused on the use of 4-NPL and related compounds as substrates for lipase and esterase activity assays. Many studies have explored the optimization of the assay conditions, the development of new assays, and the application of the assay to various biological samples. There is also research focused on the use of 4-NPL and related compounds as substrates for other enzymatic assays like protease and phosphatase assays.

Potential Implications in Various Fields of Research and Industry

The use of 4-NPL and related compounds as substrates for enzymatic assays has significant implications in various fields of research and industry. In biochemistry and molecular biology, the assay can be used to study the structure, function, and regulation of lipases and esterases. In the pharmaceutical industry, the assay can be used to screen for potential drugs that target these enzymes. In the food industry, the assay can be used to monitor the lipid content and quality of different food products.

Limitations and Future Directions

One limitation of the 4-NPL assay is that it is not specific to a particular lipase or esterase. The assay measures the activity of all lipases and esterases that can hydrolyze the substrate, which can lead to false positives or false negatives. Future research could focus on the development of more specific assays that can distinguish between different lipases and esterases.

Another limitation of the 4-NPL assay is that it does not measure the activity of lipases and esterases in their native environment. The assay is performed in vitro, and the activity of the enzyme may be influenced by factors like pH, temperature, and the presence of cofactors or inhibitors. Future research could focus on the development of assays that can measure the activity of lipases and esterases in vivo or in situ.

Future Directions

1. Development of more specific assays that can distinguish between different lipases and esterases.

2. Development of assays that can measure the activity of lipases and esterases in vivo or in situ.

3. Study of the structure, function, and regulation of lipases and esterases.

4. Screening for potential drugs that target lipases and esterases.

5. Monitoring the lipid content and quality of different food products.

6. Use of 4-NPL and related compounds as substrates for other enzymatic assays like protease and phosphatase assays.

7. Optimization of the assay conditions for improved sensitivity and accuracy.

8. Application of the assay to various biological samples for diagnostic and prognostic purposes.

9. Development of new assays that can measure the activity of lipases and esterases in complex biological samples like saliva, urine, and cerebrospinal fluid.

10. Study of the role of lipases and esterases in various physiological processes and diseases.

CAS Number1956-11-2
Product Name4-Nitrophenyl laurate
IUPAC Name(4-nitrophenyl) dodecanoate
Molecular FormulaC18H27NO4
Molecular Weight321.4 g/mol
InChIInChI=1S/C18H27NO4/c1-2-3-4-5-6-7-8-9-10-11-18(20)23-17-14-12-16(13-15-17)19(21)22/h12-15H,2-11H2,1H3
InChI KeyYNGNVZFHHJEZKD-UHFFFAOYSA-N
SMILESCCCCCCCCCCCC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]
Synonyms4-nitrophenyl laurate, p-nitrophenyl laurate, para-nitrophenyl laurate
Canonical SMILESCCCCCCCCCCCC(=O)OC1=CC=C(C=C1)[N+](=O)[O-]


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