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  •  369-07-3, ONPG, 邻硝基苯基- b -D-半乳糖苷, CAS: 369-07-3
 369-07-3, ONPG, 邻硝基苯基- b -D-半乳糖苷, CAS: 369-07-3

369-07-3, ONPG, 邻硝基苯基- b -D-半乳糖苷, CAS: 369-07-3

369-07-3, ONPG, 邻硝基苯基- b -D-半乳糖苷,
2-Nitrophenyl-β-D-galactopyranosid,
CAS: 369-07-3
C12H15NO8 / 301.25
MFCD00063255

2-Nitrophenyl b-D-galactopyranoside - non-animal origin

邻硝基苯基- b -D-半乳糖苷

o-Nitrophenyl β-D-galactopyranoside (ONPG) is a chromogenic substrate for β-galactosidase. Upon enzymatic cleavage by β-galactosidase, o-nitrophenol is released, which can be quantified by colorimetric detection at 420 nm as a measure of β-galactosidase activity.

2-nitrophenyl beta-D-galactoside is a beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position. It has a role as a chromogenic compound. It is a beta-D-galactoside and a C-nitro compound. It derives from a 2-nitrophenol.

CAS Number369-07-3
Product Name2-Nitrophenyl-beta-D-galactopyranoside
IUPAC Name(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
Molecular FormulaC₁₂H₁₅NO₈
Molecular Weight301.25 g/mol
InChIInChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChI KeyKUWPCJHYPSUOFW-UHFFFAOYSA-N
SMILESC1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Solubility45.2 [ug/mL]
Synonyms2-nitrophenyl-alpha-D-galactoside, 2-nitrophenylgalactoside, 2-nitrophenylgalactoside, (alpha-D)-isomer, 2-nitrophenylgalactoside, (beta-D)-isomer, o-nitrophenyl-beta-D-galactopyranoside, ONPG, ONPG cpd, ortho-nitrophenyl-beta-D-galactoside
Canonical SMILESC1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Isomeric SMILESC1=CC=C(C(=C1)[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O


CAS No: 369-07-3 Synonyms: ONPG MDL No: MFCD00063255 Chemical Formula: C12H15NO8 Molecular Weight: 301.25

COA:

Product name: 2-Nitrophenyl-β-D-galactopyranoside  

CAS: 369-07-3      M.F.: C12H15O8         M.W.: 301.25

Items

Standards

Results

Appearance

White or off-white crystal powder

Complies

Solubility

Soluble in DMF or hot and

insoluble in ether

Complies

Color and Turbidity

in 15mg/ml  in water

Colorless or light yellow and clear

Comply

NMR and MS

Should comply

Comply

Specific rotation

[α]20/D, in water

-68  ~  -72°

-70°

Melting point

186-193 °C (Dec.)

188-190

Carbon

46.9 - 48.8 %

Complies

Nitrogen

4.5 - 4.8 %

Complies

Loss weight on dryness

Max. 0.5%

0.11%

Residue on ignition

0.5% max

0.2%

Heavy metals

Max. 20 ppm

Complies

Assay by HPLC

Min. 98%

99.2%

Purity (Enzymatic)

Min. 98%

Complies

References:

1. Maruhn D, Clin. Chim. Acta 1976, 73, 453
2. Levvy GA, Conchie J, Methods Enzymol. 1966, 8, 571-84
3. Naider F, et al., Biochemistry 1972, 11, 3202-7

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